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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Biol Inorg Chem. 2013 Nov 12;19(1):10.1007/s00775-013-1057-6. doi: 10.1007/s00775-013-1057-6

Figure 1.

Figure 1

Effects of heat and piperidine incubations on pBR322 plasmid DNA degradation after exposure to methyl methanesulfonate (MMS). (A) Representative gel illustrating reaction of MMS (0 – 10 mM) with pBR322 DNA (0.2 mM DNA-P, 25 mM ACES, pH 7.4, 37 °C, 30 min) followed by post-treatment exposure to water (30 min, RT) (lanes 1–4), water and heat (30 min, 60 °C) (lanes 5–8) or 30 μM piperidine and heat (30 min, 60 °C) (lanes 9–12). (B) Quantification of DNA degradation as % DNA migrating as Form II for post-treatment exposure to either water (30 min, RT) (open bars); water (30 min, 60 °C) (grey bars); or 30 μM piperidine (30 min, 60 °C) (black bars). Data represent mean ± SEM for n = 5 independent experiments. Statistical significance of the effect of ± heat or ± piperidine was determined by ANOVA (NS not significant, **p<0.01, ***p<0.001, ****p<0.0001).