Figure 4.

Inner and outer retinal transduction efficiency following subretinal injection. 30 days following subretinal injection, highly efficient transduction of retinal photoreceptors was evident (A); no difference was detected in the efficiency of photoreceptor transduction between vector types (B). Similarly, cones (identified by positive cone arrestin (CAR) expression) (C) were transduced with high efficiency, but no differences were detected between vector types (D). S-cones were transduced with relatively high efficiency (E,F) white arrows in E delineate S-opsin cones with no GFP expression. There was no difference between vector types in the transduction efficiency of inner retinal cells labeled with calbindin (G, H) or calretinin (I, J). PKC-alpha positive rod bipolar cells were not transduced with great efficiency with any vector-type (K), and scAAV8(Y733F) transduced significantly fewer rod bipolar cells than the control vector scAAV5 (L). Two individual bars are shown for the control vector scAAV5 to reflect the data from the paired eye for the AAV2(quad Y-F) and the scAAV8(Y733F) groups separately. Original magnification x40 for all images. GCL ganglion cell layer; INL inner nuclear layer; ONL outer nuclear layer; RPE retinal pigment epithelium. Scale bars = 50 µm