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. Author manuscript; available in PMC: 2014 Oct 24.

Published in final edited form as: J Med Chem. 2013 Oct 8;56(20):10.1021/jm400196q. doi: 10.1021/jm400196q

Representative spectral changes (n ≥ 3) indicating trapping of NO and HNO by MbO₂ (50 μM) during decomposition at 37°C of IPA/NO-aspirin or DEA/NO-aspirin in assay buffer ± GSH (1 mM): (A) 250 μM IPA/NO-aspirin, (B) 250 μM DEA/NO-aspirin, (C) 100 μM IPA/NO-aspirin + 2% guinea pig serum and (D) 50 μM DEA/NO-aspirin (given production of 2 eqs of NO) + 2% guinea pig serum. Initial scans are in red (MbO₂), final scans without GSH are in blue (155 min for A; 350 min for B; 1170 s for C; and 600 s for D) and final scans with GSH are in green (145 min for A; 210 min for B; 1170 s for C; and 690 s for D).

Representative spectral changes (n ≥ 3) indicating trapping of NO and HNO by MbO₂ (50 μM) during decomposition at 37°C of IPA/NO-aspirin or DEA/NO-aspirin in assay buffer ± GSH (1 mM): (A) 250 μM IPA/NO-aspirin, (B) 250 μM DEA/NO-aspirin, (C) 100 μM IPA/NO-aspirin + 2% guinea pig serum and (D) 50 μM DEA/NO-aspirin (given production of 2 eqs of NO) + 2% guinea pig serum. Initial scans are in red (MbO₂), final scans without GSH are in blue (155 min for A; 350 min for B; 1170 s for C; and 600 s for D) and final scans with GSH are in green (145 min for A; 210 min for B; 1170 s for C; and 690 s for D).