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. 2013 Dec 19;2013:108486. doi: 10.1155/2013/108486

Figure 4.

Figure 4

CIG does not alter the phosphorylation level of GSK-3β and AKT. SK-N-SH cells were exposed to 10 μM WT/GFX for 3 h after the incubation with CIG (50, 100, and 200 μg/mL) for 24 h. ((a), (b)) Western blotting analysis of the levels of phosphorylated Akt at Ser473 and total Akt; ((c), (d)) western blotting analysis of the levels of phosphorylated GSK-3β at Ser9 and GSK-3β. GAPDH was used as an internal control. The level of control group was set as 100%. Data were expressed as the mean ± SD of 3 experiments. # P < 0.05 versus control group.