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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Org Chem. 2013 Oct 15;78(21):10.1021/jo401321a. doi: 10.1021/jo401321a

Figure 3.

Figure 3

Biological activity of (A) synthesized and (B) commercial (−)-Nutlin-3. Top panels: Viability test. Hs294T melanoma cells were incubated with 10μM of (−)-Nutlin-3 or vehicle control for 5 days and viable cells were counted using the trypan blue exclusion test. Bottom panels: Accumulation of p53 protein in cell lysates after 10 μM (−)-Nutlin-3 treatment, analyzed by Western Blotting. Hs294T cells were subjected to the tested compounds or vehicle control for 72 hrs. β-Actin was used as a loading control.