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. 2014 Jan 6;4:395. doi: 10.3389/fphys.2013.00395

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Copyright © 2014 Ravindran, Huang and George.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PDLSC immunohistochemistry. The fluorescent images are representative confocal micrographs of sections from PDLSC seeded scaffold explants consisting of control collagen/chitosan scaffold (A) and pulp ECM scaffold (B). The sections were analyzed for the expression of DMP1 (A1,B1), DSP (A2,B2), DPP (A3,B3), and VEGF (A4,B4). Note the absence of DSP, DPP, and VEGF signal in (A) (control scaffolds containing PDLSCs). Arrows in (B) point to positive staining of the same by PDLSCs seeded within the pulp ECM scaffold. The imaging conditions were maintained constant. The scale bar represents 20 μm in all images.