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. Author manuscript; available in PMC: 2014 Jul 2.
Published in final edited form as: Nature. 2013 Nov 17;505(7481):112–116. doi: 10.1038/nature12731

Figure 1. Nug2 binds to inter-subunit face of the pre-60S subunit clashing with export factor Nmd3.

Figure 1

(a) CRAC analyses of Nug2 and Nmd3 (performed twice; only sites were considered that were reproducibly found in both datasets). Total number of hits was plotted against the relative location along the rDNA. (b) Yeast 3-hybrid revealing interaction between Nug2 and identified 25S rRNA fragments. Negative control, empty vector and H25. (c) Nug2 (yellow) and Nmd3 binding sites (green) identified by CRAC and highlighted in the indicated 25S rRNA. (d, e) Mapping of CRAC Nug2 (yellow) and Nmd3 (green) binding sites on the 60S structure (PDB: 3O5H13). (f) Overlapping binding sites (red) of Nug2 (yellow) and Nmd3 (green).