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. 2014 Jan 1;20(1):31–41. doi: 10.1089/ars.2012.5119

FIG. 6.

FIG. 6.

Effect of endogenous H2S on cAMP rebound, AC mRNA levels in SH-SY5Y cells treated with DAMGO. (A) Western blotting analysis showing the protein expression of CBS protein in SH-SY5Y cells transfected with CBS-PME185-HA vector or its empty vector. Mean±S.E.M., n=6. *p<0.05, versus Con; #p<0.05, versus CBS. (B) Over-expression of CBS, but not its empty vector, reduced cAMP production in either forskolin-stimulated, chronic DAMGO-treated, or naloxone-precipitated cells. Mean±S.E.M., n=5–14. ***p<0.001, versus Con; #p<0.05, ##p<0.01, ###p<0.001, versus the corresponding values without NaHS treatment in the same group. (C, D) Effects of CBS over-expression on DAMGO-induced AC1 expression in SH-SY5Y cells. Mean±S.E.M, n=5. ***p<0.001, versus Con; #p<0.05, versus D. Con, control; FSK, forskolin; S, NaHS; D, DAMGO; Nal, naloxone; Veh, vehicle; Vec, vector; CBS, cystathione-β-synthase; H2S, hydrogen sulfide.