FIG. 9.

Effect of NaHS on ERK1/2 phosphorylation in the striatum of morphine-dependent mice. (A, B) Western blots showing the effects of NaHS pretreatment on naloxone-induced ERK1/2 phosphorylation in mice striatum. Mean±S.E.M, n=8. *p<0.05, versus Con; ^##p<0.01, versus Mor. (C) Immunostaining showing the effect of NaHS administration on the expression of p-ERK1/2 in mice striatum. Green: immunostaining of p-ERK1/2; Blue: the nuclei of neurons stained with Hoechst 33342. Con, control group receiving saline; Mor, morphine-treated group; S+Mor, NaHS-pretreated morphine group. Scale bar=20 μm. Con, control; S, NaHS; Mor, morphine. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars