Skip to main content
. Author manuscript; available in PMC: 2014 Jan 6.
Published in final edited form as: J Surg Oncol. 2013 Jan 17;107(7):10.1002/jso.23316. doi: 10.1002/jso.23316

Fig. 1.

Fig. 1

TAB 004 specifically detects human MUC1 in PC. A: Recombinant antibody was produced from CHO cells and antigen binding was determined against a MUC1 or control peptide via ELISA. Antibody concentration (as determined via an anti-human IgG ELISA) is plotted on the x-axis and absorbance for the ELISA is plotted on the y-axis. This assay was performed by LakePharma, San Francisco, CA. B,C: TAB 004 accurately detected MUC1 via Western blot (B) and flow cytometry (C) in KCM, BxPC3MUC1 and HPAF-II but not in KCKO and at low endogenous levels in BxPC3Neo. D: Immunohistochemistry for MUC1 using TAB 004 was performed on spontaneous pancreatic tumor in KC mice and KCM mice, which are transgenic for human MUC1, and on tumor sections from BxPC3 and HPAF-II tumors in nude mice. Representative images are displayed (n = 3 for KC, KCM and BxPC3 and n = 4 for HPAF-II).