Fig. 5.

TAB 004 detects MUC1 on PC CSCs from human patients. Patients with suspected PC that were scheduled for a Whipple procedure were consented for our IRB approved protocol. A,B: Samples were collected immediately after surgery, digested to a single-cell suspension, selected for lineage⁻ cells, dually stained with TAB 004-FITC and CD133-APC, and subjected to flow cytometry. Representative dot plots are displayed in A. CD133 levels for each patient were normalized by subjecting the isotype control levels to account for staining variability. MUC1 levels were assessed on gated CD133⁺ cells (n = 12). C: Sections from NIH tissue repository were assessed for TAB 004 and CD133 expression using immunoflourescence. TAB 004 detected MUC1 along ductal cells in the displayed lesion. Areas of TAB 004 positive cells that lacked CD133 expression were observed (green arrow). CD133 expression was also observed on the surface of ductal epithelial cells, although to a lesser extent than TAB 004. The merged image demonstrates areas that express CD133 and MUC1 as detected by TAB 004. The image is a representative of n = 6.