Fig 6.

Effect of miR-126 on the VEGF and MMP-9 expressions in hypoxia-induced RF/6A cells pretreated with miR-126-mimics or miR-126-inhibitors. The RF/6A cells were treated with miR-126-mimics, miR-126-inhibitors, or negative controls for 6 h and then kept in hypoxic incubator levels for 24 h. The protein expressions of VEGF and MMP-9 were analyzed by immunoblotting. Pretreatment with miR-126-mimics decreased VEGF and MMP-9 expression, whereas pretreatment with miR-126-inhibitors increased VEGF and MMP-9 expression in hypoxia-induced RF/6A cells. It was one representative blot of three independent experiments.