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. 2013 Dec 21;11(1):65–79. doi: 10.7150/ijms.7426

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Immunohistological staining showing that cells positive for the progenitor-specific markers Oval-6 (A), CD90 (F) and CD117 (H) were scattered in the small ductular region/canals of Hering and that these cells were not associated with bile ductule-like structures. Cells positive for the cholangiocyte-specific marker CK19 (C) were scattered in the periportal area, and some of these cells resembled the walls of bile ductules. CK8 (D) and CK18 (B) were weakly expressed in hepatocytes and strongly expressed in HPCs. The cells expressing hematopoietic cell markers CD45 (E) and CD109 (G) were located in the periportal areas and the canals of Hering. Black arrow: specific marker positive HPCs. White arrow in C: specific marker positive cells resembled bile ductule wall. White arrow in B and D: specific marker positive hepatocytes. Original magnification: 20x.