Table 6.
Microarray data validated by qRT-PCR: the fold change of mRNA expression between HPCs and hepatocytes
| Gene | Microarray | qRT-PCR | Gene Title | ||
|---|---|---|---|---|---|
| HPC | Hep | Fold (M ± SD) a | Fold (M ± SD) b | ||
| CD90 | P | P | 39.3 ±4.2 | 21.5 ± 4.8 | also known as Thy-1 |
| CK19 | P | P | 18.2 ± 2.5 | 12.5 ± 2.4 | cytokeratin 19 |
| CD45 | P | P | 6.1 ± 1.5 | 3.2 ± 0.5 | CD45 |
| CD117 | P | P | 5.2 ± 1.3 | 2.5 ± 1.4 | also known as c-kit, stem cell factor receptor |
| CK8 | P | P | 2.8 ± 0.5 | 2.1 ± 0.2 | cytokeratin 8 |
| CK18 | P | P | 1.0 ± 0.3 | 2.3 ± 0.1 | cytokeratin 18 |
| AFP | P | P | 0.3 ± 0.1 | 0.1 ± 0.01 | a-fetoprotein |
| ALB | P | P | 0.2 ± 0.01 | 0.1 ± 0.01 | albumin |
| GS | A | P | N/A | A/A | glutamine synthase |
| TAT | A | P | N/A | A/A | tyrosine aminotransferase |
| G6PD | A | P | N/A | A/A | glucose-6-phosphate dehydrogenase |
| CD34 | A | A | N/A | A/A | CD34 |
a: The fold change in mRNA expression between HPCs and hepatocytes as determined by microarray analysis was calculated from three chips in each group. b: The fold change in mRNA expression between HPCs and hepatocytes as measured by real-time RT-PCR; the results of qRT-PCR are representative of five individual samples, and PCR was performed in triplicate for each sample. A, absent; P, present; N/A, not applicable; A/A, absent from both kinds of cells.