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. 2013 Dec 21;11(1):87–96. doi: 10.7150/ijms.7651

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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The responses of T cell subsets to ConA after different MMg pre-exposure time. After 0h, 8h, 16h and 24h MMg pre-exposure, the splenocytes were transferred to a static culture condition and stimulated by ConA for 24h and 72h respectively. A) Mean fluorescence intensity (MFI) for CD25, CD69 and CD71 staining was shown gated on CD4⁺ and CD8⁺ T cells respectively. B) Percentages of cells positive for CD25, CD69 and CD71 in CD4⁺ and CD8⁺ T cell populations after a 24h-ConA stimulation. C) The FACS profile analysis of proliferation of CD4⁺ and CD8⁺ T cells after a 72h-ConA stimulation was shown. D) The proportional changes of proliferated CD4⁺ and CD8⁺ T cells after a 72h-ConA stimulation. Data were represented as means±SD. *, p<0.05, **, p<0.01 and ***, p<0.001compared with the static control group (1g).