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. 2013 Dec 21;11(1):87–96. doi: 10.7150/ijms.7651

Figure 4.

Figure 4

The cytokine production of T cell subsets and the IL-2 effect on their proliferation in response to ConA after MMg pre-exposure. After 16h-MMg pre-exposure, the splenocytes were activated with ConA at a static conditions or PMA plus Ionomycin, and then IL-2 and IFN-γ productions were investigated. A) IL-2 and IFN-γ mRNA expressions of splenocytes were evaluated after 24h-ConA or 6h-PMA/Ionomycin stimulation determined by Real-time PCR assays. B) The FACS profile analysis of IL-2 and IFN-γ intracellular staining gated on CD4+ and CD8+ T cells was shown. C) The percentages of T cell subsets positive for IL-2 and IFN-γ after 6h-PMA/Ionomycin stimulation were summarized. The FACS profile analysis (D) and the proportional changes of proliferation of CD4+ and CD8+ T cells (E) after a 72h-ConA stimulation with or without IL-2 supplying were shown. Data were represented as means±SD. *, p<0.05, **, p<0.01 and ***, p<0.001compared with the static control group (1g).