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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Cell Signal. 2013 Aug 29;25(12):10.1016/j.cellsig.2013.08.016. doi: 10.1016/j.cellsig.2013.08.016

Fig 1. Extracellular TG2 activates β-catenin signaling in Cos-7 cells.

Fig 1

(A) Dose-dependent activation of TOP-FLASH expression by purified gpTG2. (B) Exogenous recombinant human TG2 (hTG2, 100ng/mL) and overexpression of secreted human TG2 (sTG2) activate TOP-FLASH expression in Cos-7 cells. (C) Phosphorylation of the cytoplasmic domain of LRP6 is observed in LRP6-overexpressing Cos-7 cells treated for 4 hours in serum-free medium with 0.01U/mL purified gpTG2 or with 200 ng/mL canonical Wnt3a (Positive Control). (D) Treatment of Cos-7 cells with 100μM small molecule inhibitor FJ9 or overexpression of Dkk1 (30ng of expression plasmid/well) quenches activation of β-catenin by gpTG2.