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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Cell Signal. 2013 Aug 29;25(12):10.1016/j.cellsig.2013.08.016. doi: 10.1016/j.cellsig.2013.08.016

Fig 4. TG2 binds to the extracellular domain of LRP6 in vitro.

Fig 4

(A) Purified, recombinant His-tagged extracellular LRP6 domain (LRP C, 1 μg) was incubated with purified gpTG2 (1 μg). Precipitation with anti-TG2 antibody and detection by western blot with anti-His antibody showed an interaction between TG2 and LRP C. (B) Two truncated forms of TG2, TG2 C-GST and TGN-GST, or full length TG2 were incubated with LRP C. Precipitation with anti-GST antibody and detection by western blot with anti-His antibody revealed that TG2 core domain is necessary for this interaction.