Table 4.
Effects of ZnPcS 4 -AN-based PDT and/or Dox treatments on biochemical parameters of healthy and Ehrlich solid tumor-bearing mice
| G | T | Total bilirubin (mg/dL) | Direct bilirubin (mg/dL) | Indirect bilirubin (mg/dL) | AST (U/L) | ALT (U/L) | GGT (U/L) | Alkaline phosphatase (U/L) | Creatinine (mg/dL) | Urea (mg/dL) |
|---|---|---|---|---|---|---|---|---|---|---|
|
1 |
NC |
0.10 ± 0.00 |
0.04 ± 0.00 |
0.06 ± 0.00 |
71 ± 4 |
35 ± 2 |
1.88 ± 0.09 |
115 ± 9 |
0.20 ± 0.03 |
59 ± 2 |
|
2 |
CT |
0.10 ± 0.00 |
0.03 ± 0.00**
a
|
0.08 ± 0.00**
a
|
186 ± 30**
a
|
36 ± 3 |
1.8 ± 0.3 |
68 ± 8**
a
|
0.20 ± 0.08 |
57 ± 5 |
|
3 |
ZnPc |
0.10 ± 0.00 |
0.02 ± 0.00**
a
|
0.08 ± 0.00**
a
|
236 ± 30**
a
|
44 ± 3 |
2.00 ± 0.00 |
41 ± 6**
a.
*
b
|
0.16 ± 0.02 |
46 ± 3**
a
|
|
4 |
LS |
0.10 ± 0.00 |
0.02 ± 0.00**
a
|
0.08 ± 0.00**
a
|
96 ± 20*
c
|
33 ± 6 |
2.3 ± 0.3 |
42 ± 9**
a.
*
b
|
0.44 ± 0.09*
a.b.c
|
39 ± 9*
a
|
|
5 |
PDT |
0.10 ± 0.00 |
0.02 ± 0.00 |
0.08 ± 0.00**
a
|
162 ± 80 |
30 ± 9 |
1.9 ± 0.1 |
44 ± 7**
a
|
0.29 ± 0.05 |
44 ± 8 |
|
6 |
Dox |
0.10 ± 0.00 |
0.03 ± 0.00*
d
|
0.07 ± 0.00*
d
|
174 ± 20**
a
|
37 ± 4 |
1.5 ± 0.3*
c.d
|
53 ± 10 |
0.25 ± 0.06 |
55 ± 3 |
|
7 |
ZnPc/Dox |
0.10 ± 0.00 |
0.02 ± 0.00**
a
|
0.08 ± 0.00**
a
|
160 ± 10**
a
|
27 ± 2 |
1.8 ± 0.3 |
47 ± 10**
a
|
0.25 ± 0.03*
c
|
50 ± 3 |
|
8 |
Dox/LS |
0.10 ± 0.00 |
0.03 ± 0.00*
d
|
0.07 ± 0.00*
d
|
162 ± 20**
a.
*
d
|
35 ± 4 |
2.00 ± 0.00 |
88 ± 20*
c.d
|
0.18 ± 0.03 |
56 ± 5 |
|
9 |
Dox/PDT |
0.3 ± 0.2 |
0.04 ± 0.01*
d.e
|
0.2 ± 0.2 |
106 ± 20*
a.b.f.
**
c
|
25 ± 4 |
3 ± 1 |
42 ± 6 |
0.20 ± 0.02 |
39 ± 6 |
| P-values | 0.393 | 0.000 | 0.001 | 0.000 | 0.247 | 0.519 | 0.000 | 0.067 | 0.025 |
Data were expressed as mean ± SEM (standard error of mean). G = Group; T = Treatment; AST = Aspartate Aminotransferase; ALT = Alanine Aminotransferase; GGT = Gamma-Glutamyl Transferase; mg/dL = miligrams per deciliters; ULl = Units per liter. P-values of ALT were generated by ANOVA, while other values were generated by Kruskal-Wallis test. Lower-case letters indicate significant diferences in 2-by-2 comparations detected by Bonferroni (ALT) or Mann–Whitney (other variables) tests, with a = significant compared to group 1; b = significant compared to group 2; c = significant compared to group 3; d = significant compared to group 4; e = significant compared to group 5; f = significant compared to group 6. Asterisks indicate significant differences (*p < 0.05) or highly significant (**p < 0.01 or p < 0.001). For details of experimental groups see Table 5.