Table 1.
Effect of genotype and potential heterotic effects on the expression of nutrient and mineral transporter genes in the duodenal epithelium of dairy cows
| |
Genotype |
|
Significance (P-value) |
|||
|---|---|---|---|---|---|---|
| Gene name | HF | J | F 1 | SEM 1 | Genotype | HF + J vs. F 1 |
|
Nucleoside transporters
| ||||||
|
SLC28A1 |
4.39 |
4.23 |
4.50 |
0.55 |
0.95 |
0.79 |
|
SLC28A2 |
4.49 |
4.48 |
4.49 |
0.62 |
0.86 |
0.59 |
|
SLC28A3 |
4.62 |
4.76 |
5.02 |
0.48 |
0.84 |
0.59 |
|
SLC29A1 |
0.85 |
0.87 |
0.86 |
0.03 |
0.95 |
0.99 |
|
Amino acid transporters
| ||||||
|
SLC3A1 |
3.08 |
3.48 |
4.35 |
0.44 |
0.15 |
0.07 |
|
SLC3A2 |
0.98 |
1.11 |
1.28 |
0.10 |
0.13 |
0.08 |
|
SLC6A14 |
3.08 |
3.48 |
4.35 |
0.44 |
0.15 |
0.07 |
|
SLC7A1 |
1.96 |
2.04 |
2.69 |
0.45 |
0.48 |
0.24 |
|
SLC7A6 |
4.43 |
3.45 |
4.87 |
0.63 |
0.29 |
0.26 |
|
SLC7A7 |
0.95 |
0.96 |
1.17 |
0.15 |
0.54 |
0.27 |
|
SLC15A1 |
2.10 |
1.98 |
2.15 |
0.12 |
0.59 |
0.49 |
|
Sugar transporters
| ||||||
|
SLC2A2 |
6.77 |
6.09 |
6.72 |
0.93 |
0.83 |
0.81 |
|
SLC2A5 |
2.76 |
2.35 |
2.97 |
0.55 |
0.73 |
0.57 |
|
SLC5A1 |
4.75 |
3.82 |
4.74 |
0.47 |
0.29 |
0.46 |
|
Lipid transporters
| ||||||
|
ABCG8 |
4.81a |
4.66a |
8.17b |
1.24 |
0.04* |
0.02* |
|
Mineral transporters
| ||||||
|
SLC11A2 |
2.82 |
2.16 |
2.60 |
0.08 |
0.69 |
0.93 |
|
SLC31A1 |
2.48 |
2.06 |
3.59 |
1.05 |
0.61 |
0.34 |
|
SLC39A4 |
2.51 |
2.69 |
4.17 |
1.12 |
0.22 |
0.10 |
| TRPV6 | 4.02 | 3.91 | 4.57 | 0.09 | 0.92 | 0.68 |
HF = Holstein-Friesian; Je = Jersey; F1 = Holstein-Friesian x Jersey. SEM1 = pooled standard error, Genotype, *P < 0.05. Gene expression values are presented as ratios of cycle threshold (Ct) value for each gene normalized to that of the reference gene after adjustment for efficiencies and interplate variation.
a,bMeans sharing the same superscript are not significantly different at P < 0.05.
Bold represents significant (P<0.05) results in terms of difference between means in Table 1 or correlations in Tables 2 and 3.