Table 2. Semiquantitative evaluation of transduction of dog brain gray and white matters by four rAAV serotypes injected into the left cerebral hemisphere.
| Serotype | AAV2/1 | AAV2/1 | AAV2/5 | AAV2/5 | AAV2/9 | AAV2/9 | AAV2/rh10 | AAV2/rh10 |
|---|---|---|---|---|---|---|---|---|
| Dog number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| Caudate nucleus | ++ | − | − | − | ++++ | + | ++ | − |
| Thalamus | ++ | +++ | + | − | ++++ | +++ | ++++ | +++ |
| Cerebral cortex | + | ++ | + | − | ++ | ++ | − | ++ |
| Temporal lobe white matter | ++ | +++ | + | + | ++++ | +++ | +++ | +++ |
| Occipital lobe white matter | + | ++ | − | − | ++ | ++ | +++ | ++ |
| Contralateral white matter | − | − | − | − | ++ | − | − | + |
−, none.
+, sparse (<10 GFP-positive somata/ × 10 field; <5% GFP-positive axons/ × 10 field).
++, few (10–40 GFP-positive somata/ × 10 field; 5–10% GFP-positive axons/ × 10 field).
+++, moderate (40–100 GFP-positive somata/ × 10 field; 10–25% GFP-positive axons/ × 10 field).
++++, extensive (>100 GFP-positive somata/ × 10 field; >25% GFP × positive axons/ × 10 field).