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Primary neutralizing antibody responses in chicks inoculated with WNV 6-LP or 6-SP. Two-day-old (A) and 3-week-old (B) chicks (n = 3–6) were inoculated with 102 PFU of WNV 6-LP (○) or 6-SP (◆). WNV neutralizing antibody titers were then measured by PRNT80. Individual and mean PRNT80 titers are represented by symbols and bars, respectively. When mean values were calculated, the titers of samples under the detection limit were considered to be 20.
