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. Author manuscript; available in PMC: 2014 Oct 24.
Published in final edited form as: Cell. 2013 Oct 10;155(3):10.1016/j.cell.2013.09.049. doi: 10.1016/j.cell.2013.09.049

Figure 5. LKB1 Phosphorylation of AMPK is Disrupted by Cytosolic dsDNA.

Figure 5

(A) hTERT-BJ1 cells were treated with DMSO (vehicle) or compound C (10 µM) for 1 hr prior to dsDNA transfection (4 µg/ml) for the indicated times. Immunoblot was performed with the indicated antibodies.

(B) hTERT-BJ1 cells were treated with DMSO or compound C as described in Figure 5A and then transfected with dsDNA (4 µg/ml) for 6 hr. cDNA was synthesized from total RNA and then realtime PCR was performed with a probe for Ifnb.

(C) hTERT-BJ1 cells were treated with siRNA for NS (Non-specific siRNA) or LKB1 and then transfected with dsDNA (4 µg/ml) for the indicated times. Immunoblot was performed with the indicated antibodies.

(D) siRNA-treated hTERT-BJ1 cells were transfected with dsDNA (4 µg/ml) for 16 hr. IFNβ was measured by ELISA.

(E and F) hTERT-BJ1 cells were treated with compound C as described in Figure 5A (E) or siRNA for LKB1 as described in Figure 5C (F). Confocal analysis of STING trafficking in response to dsDNA (4 µg/ml) was carried out using anti-STING antibody.

Asterisks indicate significant difference (P < 0.05) compared to vehicle (B) or NS (D) determined by Student’s t-test. Error bars indicate sd. See also Figure S5.