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. Author manuscript; available in PMC: 2014 Oct 24.
Published in final edited form as: Cell. 2013 Oct 10;155(3):10.1016/j.cell.2013.09.049. doi: 10.1016/j.cell.2013.09.049

Figure 6. cGAS is Required for the Negative Regulation of STING through the AMPK-ULK1 Axis.

Figure 6

(A and B) hTERT-BJ1 cells were treated with siRNA for NS (Non-specific), STING (A), and cGAS (B) for three days and then transfected with dsDNA (4 µg/ml) for the indicated times. Immunoblot was performed with the indicated antibodies.

(C) Knock down efficiency of cGAS in hTERT-BJ1 cells were confirmed by realtime PCR.

(D) siRNA-treated hTERT-BJ1 cells were transfected with dsDNA for 6 hr as described in Figure 6A. STING trafficking was observed by confocal microscopy.

(E) siRNA-treated hTERT-BJ1 cells were transfected with dsDNA (4 µg/ml) for 16 hr. IFNβ was measured by ELISA.

Asterisks indicate significant difference (P < 0.05) compared to NS determined by Student’s t-test. Error bars indicate sd. See also Figure S6.