Fig 7.
(A) Fluorescence intensities measured from the cell lysates (λex 400 nm, λem 660 nm). MacTNPs in the cell lysates were completely de-quenched by 0.1 M NaOH/0.1% SDS solution, thereby enabling comparison of the amount of photosensitizer in the cell lysates. No significant difference in Ce6 fluorescence was observed between MacTNP-treated cells (n = 4). (B) Flow cytometric analysis of Ce6 fluorescence in HDF, non-activated and activated Raw 264.7 cells (λex 633 nm, λem 660 ± 20 nm). (C) Confocal scanning fluorescence images of the MacTNP-treated HDF, non-activated and activated macrophages (λex 633 nm, λem 700 ± 50 nm).