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. Author manuscript; available in PMC: 2014 Jan 6.
Published in final edited form as: Cancer Lett. 2010 Jul 27;298(2):10.1016/j.canlet.2010.07.003. doi: 10.1016/j.canlet.2010.07.003

Fig. 4.

Fig. 4

NAC is not a metal ionophore. A2780 cells were treated with 50 µM ZnCl2 or CuCl2 alone or in combination with 2 mM NAC for indicated times. Intracellular free zinc or copper level were assayed with the FluoZin-3® (A) and Phen Green FL (B) as described in Section 2. The cells were viewed on the Leica TCS NT Confocal Microscope using 63× Plan APO 1.2 NA (excitation 490/20 nm and emission 528/38 nm). Images are representatives of two experiments.