. Author manuscript; available in PMC: 2014 Jan 6.

Published in final edited form as: Cancer Lett. 2010 Jul 27;298(2):10.1016/j.canlet.2010.07.003. doi: 10.1016/j.canlet.2010.07.003

Table 1.

Hydrogen peroxide is generated by thiols interacting with CuCl₂. Thiols (NAC, GSH or l-cysteine) were mixed with Cu(II), Zn(II) or Fe(III) and the amount of hydrogen peroxide generated was measured after 30 min, as described in Section 2. Data are expressed as nmol of hydrogen peroxide generated in the reaction mixture (means ± SD, n = 3).

Metals (µM)		H₂O₂ (nmol/well)

		Metals alone	±NAC (2 mM)	±GSH (2 mM)	±l-Cysteine (2 mM)
CuCl₂	0	0.03 ± 0.002	0.04 ± 0.004	0.05 ± 0.001	0.07 ± 0.001
	3		0.20 ± 0.02
	10		0.28 ± 0.01
	30	0.03 ± 0.01	0.47 ± 0.01	0.41 ± 0.01	0.25 ± 0.003
	100		0.58 ± 0.007
	300	0.02 ± 0.01	0.63 ± 0.003
ZnCl₂	30	0.02 ± 0.01	0.05 ± 0.005
FeCl₃	30	0.03 ± 0.004	0.04 ± 0.003