Table 2.
Copper binding compounds attenuate the cytotoxicity of NAC/Cu(II). A2780 cells were treated with increasing concentrations of tetrathiomolybdate (TM) or bathocuproinedisulfonic acid (BCS) in the presence or absence of 1 mM NAC and 10 µM CuCl2 (dissolved in PBS buffer, added sequentially) for 72 h. Cell viability was determined by the MTS assay. Data (means ± S.D, n = 3) are expressed as percentages of the viability of untreated control cells.
| Chelators (µM) | Cell viability, % of untreated control | ||
|---|---|---|---|
| Chelator alone | +NAC (1 mM)/CuCl2 (10 µM) | ||
| TM | 0 | 100 | 2 ± 1 |
| 1 | 90 ± 11 | 2 ± 1 | |
| 3 | 78 ± 9 | 3 ± 1 | |
| 10 | 80 ± 3 | 41 ± 2 | |
| 30 | 63 ± 2 | 52 ± 5 | |
| 100 | 45 ± 7 | 57 ± 8 | |
| 300 | 35 ± 2 | 40 ± 2 | |
| BCS | 1 | 84 ± 15 | 2 ± 1 |
| 3 | 85 ± 21 | 3 ± 1 | |
| 10 | 91 ± 15 | 5 ± 1 | |
| 30 | 102 ± 20 | 9 ± 1 | |
| 100 | 110 ± 5 | 101 ± 2 | |
| 300 | 117 ± 4 | 111 ± 15 | |