Fig. 2.

PLA₂ content in BALF. (A) PLA₂ activity of the cell-free fraction of BALF was measured using a fluorescent phosphatidylcholine analog in a microplate assay format. Activity reported as average relative fluorescent units (RFU)+SE. (B) PLA₂ mRNA of the cell pellet fraction of BALF was determined by quantitative RT-PCR using primer pairs for human sPLA₂-X, sPLA₂-XII, cPLA₂-IVa, and iPLA₂-VI. Results reported in relative copy number normalized to GAPDH as average±SE; non-asthmatics (n=5), moderate-asthmatics (n=4). ^*P<0.05.