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. Author manuscript; available in PMC: 2014 Jan 6.
Published in final edited form as: Inflamm Bowel Dis. 2012 Jan 23;18(5):10.1002/ibd.22883. doi: 10.1002/ibd.22883

Figure 4. siRNA knockdown of Trib2 expression decreases phosphorylation of p38 and JNK but not p44/p42 (ERK1/2).

Figure 4

HEK293/TLR5 cells were transfected with Trib2 siRNA or control siRNA, or left untransfected (Ctrl) for 24 hours. Cells were treated with flagellin (100ng/ml) for 0, 5 or 20 min. Membranes were hybridized with indicated phospho-antibodies, then stripped and rehybridized with anti-MAPK antibodies. Bands from the 20-minute flagellin treatment were quantified by densitometry. (A, B: JNK; C, D: p38; E, F: ERK). * p<0.05 for all panels.