Figure 3.
HGF decreases effector molecules by activated CD8+ T cells. Splenocytes from Pmel-1 mice were cultured in IL-2 alone or in combination with HGF for 5 days, following antigen-specific stimulation with gp10025-33. (a and b) Flow cytometry analysis of effector cells showed that HGF decreased both (a) the number of CD8+ T cells expressing TNF, FasL, and LFA-1 and (b) the amount on a per cell basis of these molecules that play an important role in CTL cytotoxicity, as indicated by comparative geometric mean of fluorescence (GMEAN) ± SEM of three independent experiments. Final GMEAN values are the result of the ratio between the GMEAN obtained with the experimental antibody and the isotype control. Representative contour plots (a) and histograms (b) are shown. *P <0.05 by Student’s t-test. All data were obtained from three independent experiments with similar results.