Table 2.
The proteomics platform identified 21 altered proteins associated with NEC.
| spot # | Protein Name (Symbol) | Fold case/normal | p-value | Gel mass kDa/pI | Theoretical mass kDa/pI | Mascot score | % coverage | Number of unique peptides |
|---|---|---|---|---|---|---|---|---|
| 3013 | Interleukin-1 receptor antagonist (IL-1RA) | −3.15 | 0.029 | 16.23/5.01 | 20.04/5.82 | 256 | 28 | 5 |
| 2971 | −2.19 | 0.01 | 18.09/5.05 | 525 | 24 | 4 | ||
| 2892 | Peroxiredoxin-1 (Prdx1) | 4.18 | 0.004 | 23.53/8.59 | 22.09/8.27 | 274 | 39 | 8 |
| 998 | Isoform 1 of Alpha-1-antitrypsin (A1AT) | 3.18 | 0.045 | 72.77/5.53 | 46.72/5.37 | 117 | 6 | 3 |
| 1005 | 3.48 | 0.021 | 72.49/5.59 | 127 | 6 | 4 | ||
| 1203 | Clusterin isoform (apolipoprotein J) (APOJ) | −2.16 | 0.0087 | 65.50/7.62 | 52.48/5.88 | 140 | 8.91 | 3 |
| 1146 | −1.95 | 0.019 | 67.23/7.24 | 159 | 8.9 | 3 | ||
| 2777 | Proteosome subunit alpha type 2 (PSMA2) | 1.18 | 0.047 | 26.04/5.05 | 25.90/6.91 | 85 | 13.2 | 2 |
| 854 | Gelsolin (isoform 2-cytoplasmic) (GSN2) | 1.78 | 0.019 | 81.26/5.61 | 80.62/5.58 | 698 | 22 | 11 |
| 855 | 1.76 | 0.037 | 80.66/5.68 | 810 | 23 | 16 | ||
| 1897 | Cleaved Peroxisomal multifunctional enzyme type 2 (3R)-hydroxyacyl-CoA dehydrogenase) (HSD17B4) | −3.61 | 0.0016 | 38.60/8.78 | 33.59/8.6 | 298 | 9.92 | 5 |
| 2892 | Phosphatidylethanolamine-binding protein 1 (PEBP1) | 4.18 | 0.004 | 23.53/8.59 | 21.05/7.01 | 243 | 26 | 4 |
| 1975 | Alpha-2-glycoprotein 1, zinc precursor (AZGP1) | −2.24 | 0.0062 | 38.70/5.41 | 34.26/5.71 | 112 | 26 | 9 |
| 805 | Polymeric immunoglobulin receptor (PIGR) | 2.08 | 0.0026 | 86.63/5.24 | 83.260/5.59 | 238 | 7.3 | 6 |
| 2442 | cDNA FLJ75207 | −2.22 | 0.00047 | 31.15/7.35 | 29.12/8.27 | 297 | 12 | 3 |
| 2436 | −2.45 | 0.0025 | 31.12/7.0 | 326 | 12 | 4 | ||
| 3013 | Prolactin-inducible protein (PIP) | −3.15 | 0.029 | 16.23/5.01 | 16.56/8.26 | 278 | 26 | 3 |
| 3027 | −2.76 | 0.0053 | 8.85/4.63 | 172 | 4 | 2 | ||
| 1146 | Protein-glutamine gamma-glutamyltransferase E (TGM3) | −1.95 | 0.019 | 67.23/7.24 | 76.62/5.61 | 727 | 23 | 10 |
| 2892 | Neutrophil gelatinase-associated lipocalin (NGAL) | 4.18 | 0.004 | 23.53/8.59 | 22.59/9.02 | 503 | 26 | 8 |
| 2181 | N-acetylglucosamine kinase (NAGK) | −1.47 | 0.026 | 34.70/5.70 | 37.36/5.82 | 1014 | 30 | 14 |
| 1975 | cDNA FLJ53019, highly similar to Serpin B13 | −2.24 | 0.0062 | 38.70/5.41 | 45.28/5.56 | 830 | 26 | 9 |
| 1146 | cDNA FLJ54957, highly similar to transketolase | −1.95 | 0.019 | 67.23/7.24 | 68.74/7.58 | 258 | 12 | 5 |
| 1203 | −2.16 | 0.0087 | 65.50/7.62 | 222 | 8.7 | 3 | ||
| 1694 | Placental protein 11 (PP11) | −1.49 | 0.017 | 49.35/5.23 | 42.10/5.26 | 368 | 14 | 4 |
| 1173 | Isoform 1 of heat shock cognate 71 kDa protein (HSP71) | −2.37 | 0.018 | 65.92/6.3 | 70.88/5.37 | 202 | 8.2 | 4 |
| 2425 | s-formylglutathione hydrolase (esterase) (ESD) | −2.49 | 0.00027 | 31.88/6.6 | 31.45/6.54 | 86 | 7.45 | 2 |
| 2932 | Small proline-rich protein 3 (SPRR3) | 2.37 | 0.014 | 20.47/8.34 | 18.15/8.86 | 89 | 28 | 4 |
(8 increased and 13 decreased, NEC vs controls)