Table 2.
Total amino acid content in tissues of control and transgenic (K-25) L. japonicus plants that express the Ms glnβ gene (see Materials and methods). Data are the mean values in µmol g FW−1 ± SE (n = 4). The percent values that are significantly higher than the average increase are presented in bold. Asx represents a mixture of Asp/Asn, Glx represents a mixture of Glu/Gln
| Amino acid |
Leaf content (µmol g FW−1) | % Increase |
Stem content (µmol g FW−1) |
% Increase |
||
|---|---|---|---|---|---|---|
| Control | K-25 | Control | K-25 | |||
| Ala | 26.6±1.7 | 32.0±1.5 | 20 | 16.7±1.2 | 20.0 ±0.9 | 19 |
| Arg | 12.5±0.8 | 15.2±0.6 | 21 | 6.7 ±0.4 | 8.6±0.4 | 29 |
| Asx | 30.4±2.1 | 42.3 ±1.9 | 39 | 27.4 ±2.6 | 42.6 ±2.7 | 55 |
| Cys | 5.3 ±0.2 | 6.3±0.1 | 17 | 3.7±0.1 | 4.5±0.1 | 23 |
| Glx | 35.3±2.3 | 43.0 ±2.2 | 22 | 21.6±1.6 | 25.8± 1.1 | 19 |
| Gly | 28.6±1.7 | 34.3 ±1.4 | 20 | 17.9±1.2 | 21.6± 1.1 | 21 |
| His | 6.1 ±0.3 | 6.1±0.3 | 0 | 4.1 ±0.2 | 4.3±0.1 | 7 |
| Ile | 14.5±1.0 | 17.1 ±0.8 | 18 | 9.5 ±0.7 | 11.0±0.6 | 17 |
| Leu | 27.5±1.7 | 32.5±1.2 | 18 | 16.3 ±1.1 | 19.2±0.9 | 18 |
| Lys | 19.6±1.0 | 23.6 ±0.7 | 20 | 14.7± 1.0 | 17.0±0.7 | 16 |
| Met | 6.7 ±0.6 | 7.8 ±0.9 | 17 | 4.1 ±0.4 | 4.9 ±0.4 | 20 |
| Phe | 14.1 ±0.9 | 16.7±0.6 | 18 | 7.9 ±0.5 | 9.5±0.4 | 21 |
| Pro | 17.9±1.1 | 22.8 ±1.7 | 27 | 13.7± 1.2 | 18.0± 1.3 | 31 |
| Ser | 18.1 ±1.1 | 22.1 ±1.4 | 22 | 14.9± 1.1 | 17.6± 1.1 | 18 |
| Thr | 16.3±1.0 | 19.7±0.8 | 20 | 11.0±0.7 | 13.0±0.6 | 18 |
| Tyr | 0.4±0.1 | 0.5±0.2 | 12 | 1.9 ±0.2 | 1.8±0.3 | 0 |
| Val | 20.3±1.1 | 24.2 ±0.8 | 19 | 13.5±0.9 | 15.6±0.7 | 15 |
| Total | 300.3 ±18.4 | 365.9±15.8 | 22 | 205.6 ±14.8 | 255.2±12.5 | 24 |