Table 1.
Representative clones of SSH library from Boehmeria infected by C. gloeosporioides
| Clone no. | Number of identical clonesa | Best E-valueb | Identity percentage (%) | Best match in database |
|---|---|---|---|---|
| Pathogenesis-related proteins/plant defense | ||||
| 15 | emb|CT029927.1| | 8e−10 | 92 | Poplar cDNA sequences (SSH library infected with rust fungus) |
| 39 | gb|AAT02522.1| | 0.006 | 62 | Metallothionein 1a |
| 42 | gb|DQ907240.1| | 0.059 | 90 | Heat-shock transcription factor 1 |
| 46 | emb|CT029469.1| | 8e−10 | 92 | Poplar cDNA sequences (SSH library infected with rust fungus) |
| 72 | ref|XM_002519612.1| | 8e−95 | 83 | (S)-2-hydroxy-acid oxidase |
| 73 | emb|CT029702.1| | 0.063 | 100 | Poplar cDNA sequences (SSH library infected with rust fungus) |
| 88 | gb|EU311423.1| | 3e−21 | 77 | Heat-shock protein 90 mRNA |
| 141-1 | ref|XM_002273749.1| | 2e−09 | 75 | Thaumatin-like protein |
| 185 | gb|AY919870.1| | 4e−21 | 70 | Betula platyphylla metallothionein-like protein mRNA |
| 196 | emb|CU231781.1| | 6e−63 | 80 | Populus EST from mildly drought-stressed leaves |
| 205 | gb|DQ186610.1| | 3e−53 | 90 | Catharanthus roseus calmodulin 2 mRNA |
| 223 | emb|AJ007349.1|TAE7349 | 0.002 | 61 | Triticum aestivum mRNA for PR-1.2 protein |
| 235 | emb|CT028832.1| | 0.019 | 100 | Poplar cDNA sequences |
| 248 | emb|CAD42908.1| | 3e−27 | 78 | Catalase |
| 367 | gb|EU586200.1| | 4e−135 | 86 | Biquitin-conjugating enzyme 2 mRNA |
| 372 | gb|AAL36933.1|AF213481_1 | 2e−10 | 100 | Gamma-tocopherol methyltransferase |
| 425 | emb|CT028832.1| | 0.02 | 100 | Poplar cDNA sequences (SSH library infected with rust fungus) |
| 426 | gb|AY847454.1| | 1e−07 | 74 | Metallothionein class II |
| 459 | gb|EU019559.1| | 3e−52 | 83 | Glycine max WRKY14 |
| 495 | emb|CU231781.1| | 1e−54 | 76 | Populus EST from mildly drought-stressed leaves |
| 578 | gb|EU024476.1| | 1e−07 | 97 | Arachis hypogaea oxalate oxidase mRNA |
| 585 | gb|DQ497797.1| | 8e−40 | 74 | Lipoxygenase 2 |
| 589 | emb|AJ007349.1| | 4e−04 | 86 | Triticum aestivum mRNA for PR-1.2 protein |
| 599 | dbj|AB091685.1| | 3e−04 | 100 | Daidzein 7-0-methyltransferase |
| 661-2 | emb|AM748435.1| | 5e−07 | 84 | Vigna unguiculata partial mRNA for putative plant disease resistance response protein family |
| 640 | ref|XM_002519612.1| | 7e−95 | 84 | Ricinus communis (S)-2-hydroxy-acid oxidase |
| 667 | gb|AY594295.1| | 8e−17 | 68 | Populus trichocarpa × Populus deltoides metallothionein 1a (MT1a) |
| 696 | gb|EU117126.1| | 4e−24 | 81 | Prunus domestica cultivar Violette pathogenesis-related protein PR10 |
| 702 | emb|CU232440.1| | 1e−07 | 91 | Populus EST from mildly drought-stressed leaves |
| 704 | emb|AM748435.1| | 5e−12 | 91 | Putative plant disease resistance response protein family |
| 719 | emb|AJ130889.1|FSY130889 | 6e−14 | 81 | Fagus sylvatica mRNA for stress and pathogenesis-related protein |
| 761 | gb|EU526202.1| | 8e−13 | 73 | PR10 protein |
| 766 | gb|AY158153.1| | 4e−09 | 92 | Citrus × paradisi type I proteinase inhibitor |
| 792 | emb|AJ496418.1|PPE496418 | 7e−42 | 79 | Prunus persica mRNA for catalase |
| 933 | emb|CU226741.1| | 2e−89 | 83 | Populus EST from severely drought-stressed leaves |
| Metabolism | ||||
| 96 | gb|EF568776.1| | 7e−109 | 81 | Prunus persica beta-galactosidase protein |
| 288 | gb|EU106889.1| | 1e−14 | 79 | Jatropha curcas chloroplast omega-6 fatty acid desaturase mRNA |
| 600 | ref|XM_002514233.1| | 2e−51 | 77 | Ricinus communis 3-hydroxyacyl-CoA dehydrogenase |
| 664 | dbj|AB221013.2| | 5e−42 | 83 | Beta vulgaris BvSDC1 mRNA for putative serine decarboxylase |
| 707 | ref|XM_002283715.1| | 2e−25 | 85 | Vitis vinifera malate dehydrogenase (NADP+) |
| 740 | ref|XM_002515921.1| | 2e−53 | 85 | Ricinus communis malic enzyme |
| Cell growth/division | ||||
| 178 | gb|FJ226587.1| | 4e−76 | 80 | Glycine max late-embryogenesis abundant protein 2 |
| 233 | gb|FJ156098.1| | 4e−43 | 80 | Translationally controlled tumor protein |
| 587 | ref|XM_002516884.1| | 0.012 | 87 | Translationally controlled tumor protein |
| 645 | dbj|D29684.1|CUSCR9 | 4e−21 | 83 | Polypeptide for cytokinin-repressed mRNA |
| 663 | emb|AJ252088.1| | 7e−05 | 77 | mRNA for gibberellin 20-oxidase |
| 757 | emb|AJ238624.1| | 2e−89 | 88 | Translation initiation factor |
| Transporters | ||||
| 24 | ref|XM_002336761.1| | 2e−38 | 76 | Populus trichocarpa oligopeptide transporter OPT family |
| 153 | emb|AJ534339.1| | 0.008 | 86 | Agaricus bisporus partial mRNA for putative inorganic phosphate transporter (ipt gene) |
| 189 | ref|XM_002336761.1| | 8e−36 | 75 | Populus trichocarpa oligopeptide transporter OPT family |
| 457 | emb|AJ534339.1| | 3e−04 | 88 | mRNA for putative inorganic phosphate transporter |
| 660-2 | emb|AJ272202.1| | 2e−06 | 90 | Arabidopsis thaliana mRNA for mitochondrial half-ABC transporter |
| 665 | gb|EF453694.1| | 2e−70 | 78 | Populus trichocarpa Mn-specific cation diffusion facilitator transporter (MTP11.2) mRNA |
| 787 | gb|DQ372573.1| | 1e−05 | 84 | Gossypium hirsutum transferring glycosyl protein (gt) mRNA |
| Transcription | ||||
| 218 | emb|AM293617.1| | 0.075 | 100 | Platanus × acerifolia partial mRNA for putative transcription factor |
| 227 | emb|AM397241.1| | 0.006 | 93 | Ty1-copia reverse transcriptase (RT gene) and partial DfRedu pseudogene |
| 230 | dbj|AB235910.1| | 0.20 | 91 | Triticum aestivum gene for RNA ligase isoform 3 |
| 650 | emb|AM293617.1| | 0.002 | 100 | Platanus × acerifolia partial mRNA for putative transcription factor |
| 651 | gb|AF190657.1|AF190657 | 3e−39 | 83 | Nicotiana tabacum clone 7 poly(A)-binding protein (PABP) mRNA |
| 926 | gb|AY224135.1| | 7e−11 | 77 | Pelargonium × hortorum 4 coumarate CoA ligase mRNA |
| 929 | gb|AY224135.1| | 5e−12 | 75 | Pelargonium × hortorum 4 coumarate CoA ligase mRNA |
| 936-1 | gb|AY224135.1| | 2e−16 | 81 | Pelargonium × hortorum 4 coumarate CoA ligase mRNA |
| 943-1 | gb|AY224135.1| | 2e−17 | 82 | Pelargonium × hortorum 4 coumarate CoA ligase mRNA |
| 971-1 | gb|AY224135.1| | 1e−06 | 71 | Pelargonium × hortorum 4 coumarate CoA ligase mRNA |
| Signal transduction | ||||
| 205 | gb|AF292108.1|AF292108 | 3e−97 | 90 | Prunus avium calmodulin mRNA |
| 367 | gb|EU586200.1| | 4e−135 | 86 | Ubiquitin-conjugating enzyme 2 |
| 449 | ref|XM_002275581.1| | 3e−80 | 79 | Vitis vinifera ubiquitin-activating enzyme |
| 584 | ref|XM_002512452.1| | 2e−73 | 83 | Ricinus communis GTP-binding protein sar1 |
| Transposons | ||||
| 495-2 | gb|EU558534.1| | 8e−40 | 76 | Arabidopsis lyrata clone SINE9 transposon-insertion display band |
| 936-2 | gb|EU558528.1| | 1e−34 | 81 | Arabidopsis lyrata clone CACTA2 transposon-insertion display band genomic sequence |
| 944 | gb|EU558534.1| | 4e−25 | 72 | Arabidopsis lyrata clone SINE9 transposon-insertion display band genomic sequence |
| Energy | ||||
| 118 | ref|XM_002526592.1| | 8e−23 | 70 | Ricinus communis photosystem I reaction center subunit N |
| 195 | emb|AM748509.1| | 0.003 | 100 | Vigna unguiculata partial mRNA for putative single-stranded nucleic acid binding R3H |
| Protein synthesis | ||||
| 119 | ref|XM_002519706.1| | 8e−52 | 82 | Ricinus communis 60S ribosomal protein L32 |
| 194 | emb|AJ251333.1| | 4e−71 | 81 | Pisum sativum aair gene for acetohydroxy acid isomeroreductase |
| 754 | emb|AJ831469.1| | 1e−09 | 76 | Pisum sativum mRNA for putative glycine-rich protein precursor |
| 756 | emb|AJ697756.1| | 0.026 | 88 | Eucalyptus globulus subsp. globulus partial mRNA for putative ribosomal protein |
| Protein destination and storage | ||||
| 446 | emb|AM748427.1| | 0.005 | 91 | Vigna unguiculata partial mRNA for putative cathepsin B-like cysteine protease |
| Intracellular traffic | ||||
| 609 | gb|L12257.1| | 6e−71 | 82 | Glycine max nodulin-26 mRNA |
| 654 | gb|GQ354526.1| | 0.002 | 91 | Dekkera bruxellensis strain CBS 2499 mitochondrion |
| 736 | gb|L12257.1| | 7e−71 | 82 | Glycine max nodulin-26 mRNA |
| 745 | ref|XM_002522770.1| | 2e−18 | 74 | Ricinus communis mitochondrial ribosomal protein |
| Cell structure | ||||
| 204 | gb|AY487315.1| | 3e−97 | 89 | Ficus carica actin 1 mRNA |
| 943-2 | emb|AJ420893.1| | 4e−34 | 76 | Entandrophragma cylindricum microsatellite DNA |
| Secondary metabolism | ||||
| 971-2 | dbj|AB261987.1| | 1e−04 | 79 | Photinus pyralis mRNA for luciferase |
The GenBank accession number of the clones in the Table 1: GW691509-GW691606; GO653137-GO653162
aBest match in the GenBank (gb), DNA Data Bank of Japan (dbj), European Molecular Biology Laboratory (emb), or NCBI database. The accession number of the match is in parentheses
bThe expected (E) value refers to the number of matches expected by chance alone. The lower the E value, the more strongly supported the match