Skip to main content
. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Cancer Immunol Res. 2013 Apr 7;1(1):43–53. doi: 10.1158/2326-6066.CIR-13-0008

Figure 4. Trans-signaling CART cells resist AICD upon antigen specific stimulation.

Figure 4

A. Apoptosis in mixed CART cell population containing dual CAR transduced T cells (M-z/F-28), single CAR transduced T cells (M-z or F-28) and untransduced T cells that were stimulated with A1847(M+/F+) cancer cells for three days. Cis M-28z CART cells were co-cultured in parallel with A1847 for the same time period. After 3 days, apoptosis by antigen-induced cell death (AICD) was quantified using annexin V and 7-AAD staining. B. AICD is not observed in CART cells stimulated with antigen-negative cancer cells (C30; M−/F−) for 3 days.