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Optimized medium composition using different methodologies.
| Sl. no. | Concentration of component (w/%) | PGase production (U/mL) | ||||||
|---|---|---|---|---|---|---|---|---|
| χ 1 | χ 2 | χ 3 | χ 4 | χ 5 | χ 6 | Experiment | Predicated | |
| 1 | ||||||||
| Before optimization | 0.5 | 0.5 | 0.59 | 0.01 | 0.18 | 1.4 | 3.0 | — |
| 2 | ||||||||
| L27OA | 1.0 | 0.2 | 0.75 | 0.01 | 0.24 | 0.8 | 16.48 ± 0.12 | 16.52 |
| 3 | ||||||||
| RSM | 1.25 | 0.15 | 0.80 | — | — | 1.0 | 28.08 ± 0.25 | 28.41 |
| 4 | ||||||||
| ANN-GA | 0.75 | 0.20 | 0.86 | — | — | 1.1 | 31.47 ± 0.24 | 32.54 |
