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Removal of endothelium-dependent relaxation by perfusion of the Af-Art with an antibody (anti-von Willebrand factor) and complement did not alter CTGF. This suggests that the eicosanoids that mediate CTGF are released from the CNT, rather than the Af-Art endothelium. Acetylcholine failed to dilate preconstricted Af-Arts, suggesting successful endothelial removal with our antibody/complement method.
