Fig. 3.

Galactose-induced UPR activation depends on galactokinase. (A) The splicing of HAC1 mRNA was assessed by RT-PCR from strain gal1Δ grown in YPGal before and after a 2-hour treatment with 30 mM LiCl. MW, molecular weight marker. HACu indicates the product of the unspliced mRNA and HACi indicates the product of the spliced mRNA. A representative result of three independent experiments is shown. (B) Relative expression of the KAR2 gene was determined by qRT-PCR in control and gal1Δ cells grown in YPGal before and after a 2-hour treatment with 30 mM LiCl. Results are the mean ± s.d. of three independent experiments. (C,D) Similar experiments were performed using gal7Δ and gal7Δgal1Δ strains grown in YPGly medium and treated or not for 2 hours with 0.02% galactose. *P<0.05, Student’s t-test.