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. 2013 Oct 2;7(1):143–155. doi: 10.1242/dmm.012427

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© 2014. Published by The Company of Biologists Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3 — RNA foci formation in GFP(CUG)₉₁ mRNA-injected embryos. (A) In situ hybridization using a Cy5–2-O-methyl (CAG)₅ RNA probe in GFP mRNA-injected embryos at 6 hpf. There was significant GFP visible in all the cells (not shown) but no foci. (B) In situ hybridization using a Cy5–2-O-methyl (CAG)₅ RNA probe in GFP(CUG)₉₁ mRNA-injected embryos at 6 hpf. Foci were readily visible in many nuclei. In addition, there was significant diffuse nuclear and cytoplasmic Cy5 signal above that seen in GFP mRNA-injected embryos (inset). (C) Dissociated myofibers from GFP(CUG)₉₁ mRNA-injected embryos isolated at 48 hpf. Nuclear RNA foci were rarely seen, but RNA was still present diffusely in the cytoplasm and (to a lesser degree) the nucleus. (D) Dissociated myofibers from GFP(CUG)₉₁ DNA-injected embryos isolated at 48 hpf. Nuclear RNA foci were readily visible in GFP-positive myofibers, with very little visible diffusely in the nucleus or cytoplasm. Scale bars: 20 μm.