Figure 1.

Growth-inhibitory and downstream signaling effects of molecular targeted agents (NVP-AEW-541, IGFR inhibitor; MK2206, Akt inhibitor; BEZ235, PI3K/mTOR inhibitor; RAD001, mTOR inhibitor) on HCC cells and HUVECs. (A) IC₅₀ of HCC cell lines and HUVECs after drug treatments. Cells in 96-well plates were treated with drugs at the indicated concentrations for 72 h, and cell viability was assessed by MTT assay. Points, mean averages (n = 3); bars, SD. (B) Effects on Akt, GSK3β, P70S6K phosphorylation were examined by Western blotting in HCC cells and HUVECs after 24-hour drug treatments at the indicated concentrations.