Table 3.

Oligo nucleotides used for mutagenesis

Oligonucleotides	Used for	Sequence
cyoA5KO	Mutagenesis of cyoA	5′-ctcaggaaatacaataaaagtttgggatggttgtcattaattgcaggcactgcattactcagtggct gtaattctgcgctgctggatcccgtgtaggctggagctgcttc-3′
cyoA3KO	Mutagenesis of cyoA	5′-caacccttggagttggcggattccgcgtggctcatgtccataccttccattccttcatgcgagctgtgc tcaccttcaggttgggtcatgcatatgaatatcctccttag-3′
cyoB5KO	Mutagenesis of cyoB	5′-ataataagcaatcgttgcctgcgattaccctcgcagctattggggttgtctacggtgatattggt accagcccgctttatacgcttcgtgaatgtttgtcgtgtaggctggagctgcttc-3′
cyoB3KO	Mutagenesis of cyoB	5′-aaattatcactggatgcagtaccgttccatgaacctatcgtcatggtaacgatcgctgcaattatcgtc gggggactggcgatactggcagtgtaggctggagctgcttc -3′
cyoC5KO	Mutagenesis of cyoC/D mutation	5′-tgcattctgttctctattctgtttgctacctatgccgttctggtgaacggcaccgctggcggcccgacaggt aaggacattttcgaactggtgtaggctggagctgcttc -3′
cyoD3KO	Mutagenesis of cyoC/D mutation	5′-tgtagttgaggttccacataatccagatggagcccacaaccaggatggcgatgatcagcacggtaaag atgaaggccgtcatgttccagcatatgaatatcctccttag -3′
cyoA5	Confirmation of cyoA mutation	5′-atcatgtttacagtaatgta-3′
cyoA3	Confirmation of cyoA mutation	5′-tccgaacatcttatcttcct -3′
cyoB5	Confirmation of cyoB mutation	5′-aggaagataagatgttcgga-3′
cyoB3	Confirmation of cyoB mutation	5′-tcgcgtgcgttaaagtatca -3′
cyoCD5	Confirmation of cyoC/D mutation	5′-tgatactttaacgcacgcga -3′
cyoCD3	Confirmation of cyoC/D mutation	5′-tgcaggtattgcttaaacat-3′
K1	KanR primer for confirmation of mutation	5′-cagtcatagccgaatagcct-3′
K2	KanR primer for confirmation of mutation	5′-cggtgccctgaatgaactgca-3′