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. Author manuscript; available in PMC: 2014 Nov 4.
Published in final edited form as: Curr Biol. 2013 Oct 24;23(21):10.1016/j.cub.2013.08.044. doi: 10.1016/j.cub.2013.08.044

Figure 2. Tks5 Is Dispensable for Invadopodium Precursor Initiation but Is Required for Precursor Stability.

Figure 2

(A) Control or Tks5 siRNA-treated cells were stimulated with 5 nM EGF for 1 min and stained with N-WASP and cortactin antibodies to identify invadopodium precursors (shown by white arrows).

(B) Quantification of invadopodium precursors (means ± SEM) in control and Tks5 siRNA-treated cells. n = 19 (control siRNA) and 22 (Tks5 siRNA) cells.

(C) Stills from Movie S6 showing invadopodia stability. White arrows in control siRNA panel point to a stable invadopodium, whereas white arrows in Tks5 siRNA panels point to examples of unstable invadopodium precursors.

(D) Invadopodia lifetime histograms for control and Tks5 siRNA-transfected cells. Note the complete absence of stable invadopodia (lifetime > 60 min) in Tks5 siRNA cells. n = 49 (control siRNA) and 60 (Tks5 siRNA) invadopodia.

(E and F) Representative invadopodium trajectories in control (E) and Tks5 siRNA-treated (F) cells. Numbers beside each trajectory indicate lifetime (min).

(G) Quantification of invadopodium motility as net distance per lifetime (means ± SEM). n = 39 (control siRNA) and 49 (Tks5 siRNA) invadopodia.

See also Figure S2.