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. Author manuscript; available in PMC: 2014 Nov 4.
Published in final edited form as: Curr Biol. 2013 Oct 24;23(21):10.1016/j.cub.2013.08.044. doi: 10.1016/j.cub.2013.08.044

Figure 3. Binding of the PX Domain of Tks5 with PI(3,4)P2 Is Required for Invadopodium Precursor Stability and Maturation.

Figure 3

(A) Western blot showing Tks5 KD-rescue in all the four conditions.

(B–E) Stills from Movie S7 for the four Tks5 KD-rescue conditions, showing precursor stability. Note that EGF stimulates invadopodium precursor formation in all conditions (column “3 min after EGF”) but that precursors are stable only in conditions (B) and (D) and disappear in conditions (C) and (E) (column “30 min after EGF”). Scale bar represents 10 μm.

(F) Quantification of cells showing newly forming invadopodium precursors that were stable (i.e., remained until the end of the movie, 30 min). Pairwise statistical comparisons were made using Fisher’s exact test in GraphPad Prism. n = 48, 34, 37, and 59 cells analyzed for the four Tks5 KD-rescue conditions, respectively. The difference between Tks5 KD+GFP and Tks5KD+R42A/R93A was not significant (p = 0.154).

See also Figure S3.