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. Author manuscript; available in PMC: 2014 Nov 4.
Published in final edited form as: Curr Biol. 2013 Oct 24;23(21):10.1016/j.cub.2013.08.044. doi: 10.1016/j.cub.2013.08.044

Figure 5. PI(3,4)P2 Sequestration Causes Significant Reductions in Invadopodium Formation and Matrix Degradation.

Figure 5

(A) Cells were transiently transfected with GFP-TAPP1-PH, plated on 405-gelatin, and stained with cortactin and Tks5 antibodies. An area with cells having varying expression levels of TAPP1-PH is shown using rainbow lookup table (LUT). Cell outlines with no (black), low (purple), moderate (cyan), and high (red) TAPP1-PH levels are shown. Scale bar represents 10 μm.

(B and C) Scatterplots showing the effect of TAPP1-PH overexpression on cells’ ability to form invadopodia (B) and degrade matrix (C). Red lines are linear regression fit. n = 73 cells.

(D) Quantification of invadopodium precursors per cell (means ± SEM) in low (lower 25th percentile) and high (upper 25th percentile) TAPP1-PH-expressing cells, indicating that PI(3,4)P2 sequestration does not affect precursor formation. n = 20 (low) and 20 (high) cells.