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. Author manuscript; available in PMC: 2014 Nov 19.
Published in final edited form as: Biochemistry. 2013 Nov 4;52(46):10.1021/bi400816s. doi: 10.1021/bi400816s

Figure 3. Self-cleavage of the WT CPEB3 ribozyme in 0.5 mM Mg2+ and 2.5 M urea with crowding and cosolute agents.

Figure 3

PEG200, PEG8000, and Dextan10 were present at final concentrations 30% w/v. (a) Crowding and cosolute additives are present in the reaction solution prior to simultaneous addition of urea and Mg2+. (b) Crowding and cosolute additives were added 120 min after addition of urea and Mg2+, keeping the urea and Mg2+ concentration constant throughout.