Table 1.
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---|---|---|---|---|
Rg(Guinier) (Å) | Rg(GNOM) (Å) | DMax (Å) | Excluded Volume (Å3) | |
0.5 mM Mg2+ | ||||
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Buffer | 23.6 + 0.1 | 23.6 + 0.4 | 80 | 44,555 |
20% PEG200 | 26.1 + 0.5 | 25.4 ±0.5 | 87 | 45,570 |
20% PEG8000 | 21.3 + 0.1 | 21.8 + 0.7 | 75 | 40,544 |
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10 mM Mg2+ | ||||
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Buffer | 22.0 + 0.6 | 22.6 + 0.1 | 75 | 41,280 |
20% PEG8000 | 19.9 + 0.7 | 20.2 ± 0.8 | 67 | 30,877 |
Model of CPEB3 ribozyme gives Rg = 20.4 Å and Dmax=73.8 Å. The 0.5 mM Mg2+ data are at 0.6 mg/mL RNA concentration, while the 10 mM Mg2+ data in 20% PEG8000 are at 0.2 mg/mL and the 10 mM Mg2+ data in buffer alone are at ~1 mg/mL immediately following size-exclusion chromatography. The SUPCOMB20 program was used to calculate the RMSD and average excluded volume.40