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. 2014 Jan 6;9(1):e83363. doi: 10.1371/journal.pone.0083363

Figure 1. Characterization of AT-MSCs from three different donors.

Figure 1

(A) The immunophenotype of AT-MSCs from three donors was analyzed by flow cytometry. The expression of surface antigens was plotted against appropriate IgG isotype controls (black histogram). MSCs used for the analyses were positive for CD73, CD90 and CD105, and negative for CD14, CD34 and CD45 (clear histogram). The histograms presented are representative of 3 independent experiments. (B) Differentiation of AT-MSCs from three donors. Cells were incubated for 14–21 days in the presence of specific differentiation agents for osteoblasts, chondrocytes, and adipocytes. Alkaline phosphatase staining shows mineralization of the extracellular matrix. Toluidine Blue staining shows the deposition of proteoglycans and lacunae. Differentiation into the adipocyte lineage was demonstrated by staining with Oil Red O. (Magnification: ×100).