Table 3. Supplements that were spotted on 5Clostridium difficile base agar.

Supplement	Concentration	Amount (µl)	VLOO13	CD26A54_S	CD26A54_R
L-cysteine HCl	50 mg/ml	20	+	+	−
5% Laked sheep blood	undiluted	20	+	+	+
Vitamin K	10 mg/ml	20	+	+	−
L-homoserine	50 mg/ml	20	+	+	−
Thiamine pyrophosphate	100 µg/ml	20	+	+	−
Fetal bovine serum	undiluted	20	+	+	−
Casamino acids	7%	20	+	+	−
FeCl2	1 mg/ml	20	+	+	+
Hemin	5 mg/ml	20	+	+	+

(+), growth. (−), no growth.