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. 2014 Jan 6;9(1):e83385. doi: 10.1371/journal.pone.0083385

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© 2014 Sakakura et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, Expression of CD109 in total cell lysates and detection of soluble CD109 in culture media of HEK293-FLAG-hCD109 and -VC cell line. Total cell lysates and culture media were subjected to western blotting using the indicated antibodies; blots of anti-β-actin antibody shown as internal controls. B, Cell proliferation analysis of HEK293-FLAG-hCD109 and -VC cell lines. Absorbance values at day 1 are defined as 1.0. NS: not significant, using the Tukey–Kramer's HSD test.