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. 2014 Jan 6;9(1):e84416. doi: 10.1371/journal.pone.0084416

Figure 4. Effect of miR398b over-expression in transgenic roots from composite plants grown under CuD.

Figure 4

Composite plants were obtained through A. rhizogenes transformation with EV or with OE398 plasmid, these were grown in control (sufficient nutrient) condition (C) or in CuD stress condition. (A) Relative expression of miR398b (blue) and of (B) target genes CSD1 (green) and Nod19 (red) determined by qRT-PCR; values were normalized to the value from the EV roots grown in the C condition that was set to 1. (C) Anthocyanin contents in root crown of composite plants. (D) Expression ratio (CuD:C) of copper-stress responsive genes: Fe superoxide dismutase (FSD, yellow), high affinity Cu transporter (COPT, purple) and ferric chelate reductase (FRO, brown). Values represent the average ± SD from three biological replicates.